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matchmaker gold yeast  (TaKaRa)


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    TaKaRa matchmaker gold yeast
    Matchmaker Gold Yeast, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 2372 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/matchmaker gold yeast/product/TaKaRa
    Average 97 stars, based on 2372 article reviews
    matchmaker gold yeast - by Bioz Stars, 2026-05
    97/100 stars

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    Protein interactions between UvBhlh6 and other bHLH transcription factors. (a) <t>Yeast</t> <t>two‐hybrid</t> assay assessing interactions between UvBhlh6 and UvBhlh1, UvBhlh4 and UvBhlh7. Yeast cells (10 2 –10 6 cells/mL) co‐transformed with prey and bait vectors were spotted on SD/−Leu/−Trp and SD/−Ade/−His/−Leu/−Trp media. Positive control: PGADT7 (AD‐T) + pGBKT7‐53 (BD‐53); negative control: PGADT7 (AD‐T) + pGBKT7‐Lam (BD‐Lam). (b) Bimolecular fluorescence complementation assay detecting interactions between UvBhlh6 and UvBhlh1, UvBhlh4 or UvBhlh7. YFP signals and DAPI staining were visualised using an LSM710 confocal microscope (Zeiss). Scale bar, 10 μm.
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    Protein interactions between UvBhlh6 and other bHLH transcription factors. (a) <t>Yeast</t> <t>two‐hybrid</t> assay assessing interactions between UvBhlh6 and UvBhlh1, UvBhlh4 and UvBhlh7. Yeast cells (10 2 –10 6 cells/mL) co‐transformed with prey and bait vectors were spotted on SD/−Leu/−Trp and SD/−Ade/−His/−Leu/−Trp media. Positive control: PGADT7 (AD‐T) + pGBKT7‐53 (BD‐53); negative control: PGADT7 (AD‐T) + pGBKT7‐Lam (BD‐Lam). (b) Bimolecular fluorescence complementation assay detecting interactions between UvBhlh6 and UvBhlh1, UvBhlh4 or UvBhlh7. YFP signals and DAPI staining were visualised using an LSM710 confocal microscope (Zeiss). Scale bar, 10 μm.
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    Protein interactions between UvBhlh6 and other bHLH transcription factors. (a) Yeast two‐hybrid assay assessing interactions between UvBhlh6 and UvBhlh1, UvBhlh4 and UvBhlh7. Yeast cells (10 2 –10 6 cells/mL) co‐transformed with prey and bait vectors were spotted on SD/−Leu/−Trp and SD/−Ade/−His/−Leu/−Trp media. Positive control: PGADT7 (AD‐T) + pGBKT7‐53 (BD‐53); negative control: PGADT7 (AD‐T) + pGBKT7‐Lam (BD‐Lam). (b) Bimolecular fluorescence complementation assay detecting interactions between UvBhlh6 and UvBhlh1, UvBhlh4 or UvBhlh7. YFP signals and DAPI staining were visualised using an LSM710 confocal microscope (Zeiss). Scale bar, 10 μm.

    Journal: Molecular Plant Pathology

    Article Title: The Basic Helix–Loop–Helix Transcription Factors UvBhlh1 and UvBhlh6 Coordinate Virulence in the Rice False Smut Fungus Ustilaginoidea virens

    doi: 10.1111/mpp.70267

    Figure Lengend Snippet: Protein interactions between UvBhlh6 and other bHLH transcription factors. (a) Yeast two‐hybrid assay assessing interactions between UvBhlh6 and UvBhlh1, UvBhlh4 and UvBhlh7. Yeast cells (10 2 –10 6 cells/mL) co‐transformed with prey and bait vectors were spotted on SD/−Leu/−Trp and SD/−Ade/−His/−Leu/−Trp media. Positive control: PGADT7 (AD‐T) + pGBKT7‐53 (BD‐53); negative control: PGADT7 (AD‐T) + pGBKT7‐Lam (BD‐Lam). (b) Bimolecular fluorescence complementation assay detecting interactions between UvBhlh6 and UvBhlh1, UvBhlh4 or UvBhlh7. YFP signals and DAPI staining were visualised using an LSM710 confocal microscope (Zeiss). Scale bar, 10 μm.

    Article Snippet: Protein–protein interactions were analysed using the Matchmaker Gold Yeast Two‐Hybrid System (Clontech).

    Techniques: Y2H Assay, Transformation Assay, Positive Control, Negative Control, Bimolecular Fluorescence Complementation Assay, Staining, Microscopy

    Subcellular localisation and protein interactions involving UvBhlh6. (a) Co‐localisation of DAPI and UvBhlh1‐eGFP, UvBhlh4‐eGFP, UvBhlh6‐eGFP and UvBhlh7‐eGFP in mycelia. Scale bar, 10 μm. (b) Yeast two‐hybrid assay assessing interactions between UvBhlh6 and its putative interacting partners identified by affinity capture‐mass spectrometry. Yeast cells were spotted on SD/−Leu/−Trp and SD/−Ade/−His/−Leu/−Trp media. Controls: Positive, pGADT7 (AD‐T) + pGBKT7‐53 (BD‐53); negative, pGADT7 (AD‐T) + pGBKT7‐Lam (BD‐Lam).

    Journal: Molecular Plant Pathology

    Article Title: The Basic Helix–Loop–Helix Transcription Factors UvBhlh1 and UvBhlh6 Coordinate Virulence in the Rice False Smut Fungus Ustilaginoidea virens

    doi: 10.1111/mpp.70267

    Figure Lengend Snippet: Subcellular localisation and protein interactions involving UvBhlh6. (a) Co‐localisation of DAPI and UvBhlh1‐eGFP, UvBhlh4‐eGFP, UvBhlh6‐eGFP and UvBhlh7‐eGFP in mycelia. Scale bar, 10 μm. (b) Yeast two‐hybrid assay assessing interactions between UvBhlh6 and its putative interacting partners identified by affinity capture‐mass spectrometry. Yeast cells were spotted on SD/−Leu/−Trp and SD/−Ade/−His/−Leu/−Trp media. Controls: Positive, pGADT7 (AD‐T) + pGBKT7‐53 (BD‐53); negative, pGADT7 (AD‐T) + pGBKT7‐Lam (BD‐Lam).

    Article Snippet: Protein–protein interactions were analysed using the Matchmaker Gold Yeast Two‐Hybrid System (Clontech).

    Techniques: Y2H Assay, Mass Spectrometry